97影院,99香蕉国产精品偷在线观看,亚洲成a人片在线播放,久久夜色精品国产噜噜麻豆

您好,歡迎進入研域(上海)化學試劑有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
  • 技術(shù)文章ARTICLE

    您當前的位置:首頁 > 技術(shù)文章 > 心肌營養(yǎng)素1(CT1)ELISA試劑盒英文說明書

    心肌營養(yǎng)素1(CT1)ELISA試劑盒英文說明書

    發(fā)布時間: 2017-05-21  點擊次數(shù): 1516次

    心肌營養(yǎng)素1(CT1)ELISA試劑盒
    適用生物 Homo sapiens (Human,人)
    檢測范圍 15.63-1000pg/mL 靈敏度 5.5pg/mL
    樣本類型 Serum, plasma and other biological fluids.
    實驗時長 4.5h 實驗方法 雙抗夾心法 

    心肌營養(yǎng)素1(CT1)ELISA試劑盒    規(guī)格 96T
    ELISA Kit for Cardiotrophin 1 (CT1)
    FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
    Organism species Homo sapiens (Human)
    Product No. SEA810Hu
    Sample type Serum, plasma and other biological fluids.

    心肌營養(yǎng)素1(CT1)ELISA試劑盒    Format 96-well strip plate
    Assay length 4.5 hours
    Detection range 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL 
    Sensitivity The minimum detectable dose of this kit is typically less than 5.5pg/mL.
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Cardiotrophin 1 (CT1).
    No significant cross-reactivity or interference between Cardiotrophin 1 (CT1) and analogues was observed. Recovery
    Matrices listed below were spiked with certain level of recombinant Cardiotrophin 1 (CT1) and the recovery rates were calculated by comparing the measured value to the expected amount of Cardiotrophin 1 (CT1) in samples.
    Matrix Recovery range (%) Average(%)
    serum(n=5) 82-103 101
    EDTA plasma(n=5) 95-102 99
    heparin plasma(n=5) 85-99 93
    Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    心肌營養(yǎng)素1(CT1)ELISA試劑盒     Linearity
    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cardiotrophin 1 (CT1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Sample 1:2 1:4 1:8 1:16
    serum(n=5) 81-88% 81-92% 96-104% 78-104%
    EDTA plasma(n=5) 78-97% 93-105% 78-97% 84-92%
    heparin plasma(n=5) 94-105% 78-102% 88-102% 79-101%
    Stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Reagents and materials provided
    Reagents Quantity Reagents Quantity
    Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
    Standard 2 Standard Diluent 1×20mL
    Detection Reagent A 1×120μL Assay Diluent A 1×12mL
    Detection Reagent B 1×120μL Assay Diluent B 1×12mL
    TMB Substrate 1×9mL Stop Solution 1×6mL
    Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
    Assay procedure summary
    1. Prepare all reagents, samples and standards;
    2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
    4. Aspirate and wash 3 times;
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
    6. Aspirate and wash 5 times;
    7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
    8. Add 50μL Stop Solution. Read at 450nm immediay.

    心肌營養(yǎng)素1(CT1)ELISA試劑盒    Test principle
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cardiotrophin 1 (CT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cardiotrophin 1 (CT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cardiotrophin 1 (CT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cardiotrophin 1 (CT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

產(chǎn)品中心 Products
在線客服 聯(lián)系方式

服務(wù)熱線

021-54479081
021-54461587

大黑鸡巴操逼舔屄视频| 免费亚洲一级色片| 美女叉开腿让男人捅一二三区精选| 国产人妻人伦精品一区二区网站1| 日韩视频视频| 自拍偷拍制服丝袜中文字幕| 啊啊啊操bav| 一级A A网站| 国产传媒一区二区三区四区| 亚洲人一区四区蜜桃| 91麻豆污污污| 91AV在线观看蜜臀| 成人视频四区五区| 红桃视频免费看黄片日韩| 美女插鸡巴视频网站 | 操五常市女人逼| 国产偷人视频免费| AV一一本道色色| 久久成人在线视频| 手机看片偷拍福利大屁股| 操大鸡吧电影| 去干网在线观看| 激情文学蜜桃视频| 亚洲AV成人精品午夜一区二区| 日韩偷拍人体| 蜜桃视频第一区| 欧美骚女视频| 日本少妇大奶一级片一区二区| 欧洲一区裸体视频| 视频一区美国妞人妻熟女| 嗯啊嗯嗯啊不要啊阿视频 | 操骚逼利视频| 国产激情 视频一| 手机看片大香蕉在线视频| 九九热偷拍视频| 午夜福利第一区| 十八禁视频道网站| 免费无码日逼| 老逼高清无码| 岛国精品视频免费看| 熟妇熟女五十路又大又粗|