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細(xì)胞中文名稱(chēng)正常小鼠睪丸Leydig細(xì)胞
細(xì)胞英文名稱(chēng)TM3
物種小鼠
細(xì)胞形態(tài)特征上皮樣
細(xì)胞生長(zhǎng)特性正常小鼠睪丸Leydig細(xì)胞TM3細(xì)胞 貼壁生長(zhǎng)
細(xì)胞特種特性 該細(xì)胞在LH的作用下cAMP產(chǎn)量增加,但對(duì)FSH無(wú)反應(yīng);該細(xì)胞對(duì)LH反應(yīng)性的維持依賴于血清批號(hào);在LH存在的情況下,該細(xì)胞可代謝膽固醇;可產(chǎn)生前列腺素F2a;表達(dá)LH受體、EGF受體、AR、ER和PR。鼠痘病毒陰性
培養(yǎng)基DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium)
血清5%HS + 2.5%FBS
細(xì)胞傳代方法1:20~1:200傳代,每3~4天換液1次
細(xì)胞傳代情況C2
細(xì)胞凍存條件基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
支原體檢測(cè)陰性
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