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人白介素1可溶性受體Ⅰ酶免試劑盒,(IL-1sRⅠ)ELISA檢測(cè)試劑盒,英文名:IL-1sRⅠ ELISA Kit,英文縮寫:IL-1sRⅠ ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人白介素1可溶性受體ⅠELISA檢測(cè)試劑盒,IL-1sRⅠ 檢測(cè)試劑盒,IL-1sRⅠ 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。人白介素1可溶性受體Ⅰ酶免試劑盒,(IL-1sRⅠ)ELISA檢測(cè)試劑盒 操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
兔熱休克蛋白20(HSP-20)ELISA試劑盒Rabbit Heat Shock Protein 20,HSP-20 ELISA KitY0315196T/48T
兔β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒Rabbit amyloid beta peptide 1-40,Aβ1-40 ELISA KitY0315296T/48T
兔組織因子途徑抑制物(TFPI)ELISA試劑盒Rabbit Tissue factor pathway inhibitor,TFPI ELISA KitY0315396T/48T
兔白介素17(IL-17)ELISA試劑盒Rabbit Interleukin 17,IL-17 ELISA KitY0315496T/48T
兔乙酰膽堿(ACh)ELISA試劑盒Rabbit acetylcholine,ACH ELISA KitY0315596T/48T
兔去甲腎上腺素(NA)ELISA試劑盒Rabbit Noradrenaline,NA ELISA KitY0315696T/48T
兔子生長激素釋放多肽(GHRP)ELISA試劑盒Rabbit Growth Hormone Releasing Peptide,GHRP ELISA Kit Y0315796T/48T
兔子α干擾素(IFN-α)ELISA試劑盒 Rabbit Interferon α,IFN-α ELISA Kit Y0315896T/48T
兔子E選擇素(E-Selectin/CD62E)ELISA試劑盒 Rabbit E-Selectin ELISA Kit Y0315996T/48T
兔子視黃醇結(jié)合蛋白4(RBP-4)ELISA試劑盒Rabbit Retinol binding protein 4,RBP-4 ELISA Kit Y0316096T/48T
兔子主要組織相容性復(fù)合體Ⅲ類(MHCⅢ/RLAⅢ)ELISA試劑盒Rabbit major histocompatibility complex Ⅲ,MHCⅢ/RLAⅢ ELISA Kit Y0316196T/48T
兔淋巴細(xì)胞功能相關(guān)抗原3(LFA-3/CD58)ELISA試劑盒Rabbit lymphocyte function associated antigen 3,LFA-3 ELISA Kit Y0316296T/48T
兔兒茶酚胺(CA)ELISA試劑盒Rabbit catecholamine,CA ELISA Kit Y0316396T/48T
兔吡啶交聯(lián)物(PY)ELISA試劑盒Rabbit pyridinium crosslink/PyriLinks,PY ELISA Kit Y0316496T/48T
兔糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒Rabbit Glycated hemoglobin A1c,GHbA1c ELISA Kit Y0316596T/48T
兔半胱氨酸蛋白酶抑制劑/胱抑素C(Cys-C)ELISA試劑盒Rabbit Cystatin C,Cys-C ELISA Kit Y0316696T/48T
兔子脫氧吡啶酚/脫氧吡啶啉(DPD)ELISA試劑盒deoxypyridinoline,DPD ELISA Kit Y0316796T/48T
兔子組織因子(TF)ELISA試劑盒rabbit Tissue factor,TF ELISA KitY0316896T/48T
兔子骨膠原交聯(lián)(Cr)ELISA試劑盒Rabbit Crosslaps,Cr ELISA Kit Y0316996T/48T
兔子組織型纖溶酶原激活劑(t-PA)ELISA試劑盒 rabbit Tissue-type Plasiminogen Actilyse,t-PA ELISA KitY0317096T/48T
兔子組織多肽抗原(TPA)ELISA試劑盒rabbit Tissue Polypeptide Antigen,TPA ELISA Kit Y0317196T/48T
兔子組胺(HIS)ELISA試劑盒rabbit Histamine,HIS ELISA KitY0317296T/48T
兔子轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒rabbit Transforming Growth factor β1,TGF-β1 ELISA KitY0317396T/48T
兔子腫瘤壞死因子α(TNF-α) ELISA試劑盒 rabbit Tumor necrosis factor α,TNFα ELISA Kit Y0317496T/48T
兔子脂聯(lián)素(ADP)ELISA試劑盒rabbit adiponectin,ADP ELISA KitY0317596T/48T
兔腎素(Renin)ELISA試劑盒Rabbit Renin ELISA Kit Y0317696T/48T
兔子胰島素樣生長因子2(IGF-2)ELISA試劑盒rabbit Insulin-like growth factor 2,IGF-2 ELISA Kit Y0317796T/48T
兔子胰島素樣生長因子1(IGF-1)ELISA試劑盒rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit Y0317896T/48T
兔子胰島素(INS)ELISA試劑盒rabbit Insulin,INS ELISA Kit Y0317996T/48T
兔子氧化低密度脂蛋白(OxLDL)ELISA試劑盒Rabbit oxidized lowdensity lipoprotein,OxLDL ELISA Kit Y0318096T/48T
兔子補(bǔ)體蛋白4(C4)ELISA試劑盒Rabbit Complement 4,C4 ELISA Kit Y0318196T/48T
兔子血小板衍生生長因子(PDGF)ELISA試劑盒rabbit plaet-derived growth factor,PDGF ELISA Kit Y0318296T/48T
兔過氧化物酶體增殖因子活化受體γ(PPAR-γ)ELISA試劑盒Rabbit Peroxisome Proliferator-activated receptor γ,PPAR-γ ELISA Kit Y0318396T/48T
兔子血小板活化因子(PAF)ELISA試劑盒rabbit plaet activating factor,PAF ELISA Kit Y0318496T/48T
兔子血栓調(diào)節(jié)蛋白(TM)ELISA試劑盒 rabbit thrombomodulin,TM ELISA Kit Y0318596T/48T
兔子血纖蛋白原降解產(chǎn)物(FDP)ELISA試劑盒rabbit Fibrinogen Degradation Product,FDP ELISA Kit Y0318696T/48T
兔子纖溶酶原激活物抑制因子(PAI)ELISA試劑盒rabbit plasminogen activator inhibitor,PAI ELISA Kit Y0318796T/48T
兔子纖溶酶原激活物抑制因子1(PAI-1)ELISA試劑盒rabbit plasminogen activator inhibitor 1,PAI-1 ELISA Kit Y0318896T/48T
兔子細(xì)胞間粘附分子3(ICAM-3/CD50)ELISA試劑盒rabbit intercellular adhesion molecule 3,ICAM-3 ELISA Kit Y0318996T/48T
兔子葡萄糖依賴性胰島素釋放多肽(GIP)ELISA試劑盒Rabbit Glucose dependent insulin releasing polypeptide,GIP ELISA Kit Y0319096T/48T
兔子細(xì)胞間粘附分子2(ICAM-2/CD102)ELISA試劑盒rabbit intercellular adhesion molecule 2,ICAM-2 ELISA KitY0319196T/48T
兔子細(xì)胞間粘附分子1(ICAM-1/CD54)ELISA試劑盒rabbit intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit Y0319296T/48T
兔子透明質(zhì)酸(HA)ELISA試劑盒rabbit Hyaluronic acid,HA ELISA Kit Y0319396T/48T
兔子瘦素(LEP)ELISA試劑盒rabbit Leptin,LEP ELISA Kit Y0319496T/48T
兔子神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒rabbit neurotrophin 4,NT-4 ELISA Kit Y0319596T/48T
兔子神經(jīng)營養(yǎng)因子3(NT-3)ELISA試劑盒rabbit neurotrophin 3,NT-3 ELISA Kit Y0319696T/48T
兔子神經(jīng)特異性烯醇化酶(NSE)ELISA試劑盒rabbit Neuron-specific enolase,NSE ELISA KitY0319796T/48T
兔子神經(jīng)生長因子(NGF)ELISA試劑盒 rabbit Nerve growth factor,NGF ELISA KitY0319896T/48T
兔子髓磷脂堿性蛋白(MBP)ELISA試劑盒Rabbit myelin basic protein,MBP ELISA Kit Y0319996T/48T
兔子前列腺素E2(PGE2)ELISA試劑盒rabbit Prostaglandin E2,PG-E2 ELISA KitY0320096T/48T
各種類型ELISA測(cè)定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。