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人乙型肝炎表面抗原酶免試劑盒,(HBsAg)ELISA檢測試劑盒,英文名:HBsAg ELISA Kit,英文縮寫:HBsAg ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人乙型肝炎表面抗原ELISA檢測試劑盒,HBsAg 檢測試劑盒,HBsAg 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人乙型肝炎表面抗原酶免試劑盒,(HBsAg)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
凋亡蛋白酶激活因子1(APAF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apoptotic 表面活性物質(zhì)關(guān)聯(lián)蛋白C(SPC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Surfactant Associated Protein C (SPC)48T/96TY09035Homo sapiens (Human)
二胺氧化酶(DAO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Diamine Oxidase (DAO)48T/96TY09036Homo sapiens (Human)
淋巴細(xì)胞功能關(guān)聯(lián)抗原1α(LFA1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lymphocyte Function Associated Antigen 1 Alpha (LFA1a)48T/96TY09037Homo sapiens (Human)
防御素α1(DEFα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Defensin Alpha 1, Neutrophil (DEFa1)48T/96TY09038Homo sapiens (Human)
血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY09039Canis familiaris; Canine (Dog)
脂質(zhì)運載蛋白1(LCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipocalin 1 (LCN1)48T/96TY09040Homo sapiens (Human)
半胱氨酰白三烯受體2(CYSLTR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cysteinyl Leukotriene Receptor 2 (CYSLTR2)48T/96TY09041Rattus norvegicus (Rat)
Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 4 (TLR4)48T/96TY09042Homo sapiens (Human)
天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aspartate Aminotransferase (AST)48T/96TY09043Rattus norvegicus (Rat)
天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aspartate Aminotransferase (AST)48T/96TY09044Mus musculus (Mouse)
轉(zhuǎn)錄激活因子5(ATF5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activating Transcription Factor 5 (ATF5)48T/96TY09045Rattus norvegicus (Rat)
白介素17F(IL17F)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17F (IL17F)48T/96TY09046Homo sapiens (Human)
血小板激活因子乙酰水解酶2(PAFAH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Activating Factor Acetylhydrolase 2 (PAFAH2)48T/96TY09047Homo sapiens (Human)
血管內(nèi)皮生長因子受體1(VEGFR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 1 (VEGFR1)48T/96TY09048Mus musculus (Mouse)
Salusinβ蛋白(Salusinβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Salusin Beta48T/96TY09049Homo sapiens (Human)
O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT)48T/96TY09050Homo sapiens (Human)
白介素28B(IL28B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 28B (IL28B)48T/96TY09051Homo sapiens (Human)
白介素29(IL29)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 29 (IL29)48T/96TY09052Homo sapiens (Human)
腦型肌酸激酶(CKB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Creatine Kinase, Brain (CKB)48T/96TY09053Mus musculus (Mouse)
白介素4受體(IL4R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 4 Receptor (IL4R)48T/96TY09054Homo sapiens (Human)
分泌性白細(xì)胞蛋白酶抑制因子(SLPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)48T/96TY09055Rattus norvegicus (Rat)
載脂蛋白C4(APOC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein C4 (APOC4)48T/96TY09056Homo sapiens (Human)
胰島素樣生長因子1(IGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY09057Rhesus monkey (Simian)
CD14分子(CD14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 14 (CD14)48T/96TY09058Homo sapiens (Human)
腦啡肽(ENK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Enkephalin (ENK)48T/96TY09059Rattus norvegicus (Rat)
甲胎蛋白異質(zhì)體3(αFPL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein Lens Culinaris Agglutinin 3 (aFPL3)48T/96TY09060Homo sapiens (Human)
金屬硫蛋白1(MT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Metallothionein 1 (MT1)48T/96TY09061Homo sapiens (Human)
精氨酸酶(Arg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arginase (Arg)48T/96TY09062Homo sapiens (Human)
巨噬細(xì)胞刺激蛋白(MSP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Stimulating Protein (MSP)48T/96TY09063Homo sapiens (Human)
Opiorphin蛋白(OPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Opiorphin (OPI)48T/96TY09064Homo sapiens (Human)
著絲粒蛋白B(CENPB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Centromere Protein B (CENPB)48T/96TY09065Homo sapiens (Human)
脫氧核糖核酸酶Ⅰ(DNASE1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Deoxyribonuclease I (DNASE1)48T/96TY09066Homo sapiens (Human)
小核糖核蛋白多肽D1(SNRPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1)48T/96TY09067Homo sapiens (Human)
抗核糖核蛋白抗體(Anti-RNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Ribonucleoprotein Antibody (Anti-RNP)48T/96TY09068Homo sapiens (Human)
抗Jo1抗體(Anti-Jo1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Jo1 Antibody48T/96TY09069Homo sapiens (Human)
抗Scl-70抗體(Anti-Scl70)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Scl 70 Antibody48T/96TY09070Homo sapiens (Human)
抗肝腎微粒體抗體(Anti-LKMA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Liver/Kidney Microsomal Antibody (Anti-LKMA)48T/96TY09071Homo sapiens (Human)
絲裂原激活蛋白激酶激酶激酶激酶5(MAP4K5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5)48T/96TY09072Homo sapiens (Human)
環(huán)胍氨酸肽(CCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclical Citrullinated Peptide (CCP)48T/96TY09073Homo sapiens (Human)
造骨細(xì)胞鈣黏蛋白(CDHOB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin, Osteoblast (CDHOB)48T/96TY09074Homo sapiens (Human)
抗絲聚蛋白(AFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Filaggrin Antibody (AFA)48T/96TY09075Homo sapiens (Human)
抗利尿激素(ADH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Antidiuretic Hormone (ADH)48T/96TY09076Homo sapiens (Human)
抗卵巢抗體(AOAb)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Ovarian Antibody (AOAb)48T/96TY09077Homo sapiens (Human)
抗Ⅱ型膠原蛋白抗體(Anti-CⅡAb)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Type II Collagen Antibody (Anti-CIIAb)48T/96TY09078Homo sapiens (Human)
153kDa核孔蛋白(NUP153)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 153kDa (NUP153)48T/96TY09079Homo sapiens (Human)
絲裂原激活蛋白激酶激酶激酶1(MAP3K1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase Kinase Kinase 1 (MAP3K1)48T/96TY09080Homo sapiens (Human)
抗OJ抗體(Anti-OJ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-OJ Antibody48T/96TY09081Homo sapiens (Human)
抗組蛋白抗體(AHA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Histone Antibody (AHA)48T/96TY09082Homo sapiens (Human)
磷脂酶A1(PLA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A1 (PLA1)48T/96TY09083Homo sapiens (Human)
133kDa核孔蛋白(NUP133)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 133kDa (NUP133)48T/96TY09084Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。