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您當(dāng)前的位置:首頁 > 產(chǎn)品展示 > ELISA檢測試劑盒 > 小鼠ELISA檢測試劑盒 > 小鼠基質(zhì)金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒
小鼠基質(zhì)金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒
產(chǎn)品時間:2016-10-11
小鼠基質(zhì)金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒,行業(yè)*,國內(nèi)外多家ELISA試劑盒代理供應(yīng),價格相對平價,公司提供免費代測及ELISA試劑盒包郵服務(wù)。

本產(chǎn)品僅用于科研,不用于臨床

小鼠基質(zhì)金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒,英文名:MMP-2/Gelatinase A ELISA Kit,英文縮寫:MMP-2/Gelatinase A ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠基質(zhì)金屬蛋白酶2/明膠酶AELISA檢測試劑盒,MMP-2/Gelatinase A 檢測試劑盒,MMP-2/Gelatinase A 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠基質(zhì)金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。

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轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)48T/96TY11561Bos taurus; Bovine (Cattle)
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組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11572Bos taurus; Bovine (Cattle)
組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11573Sus scrofa; Porcine (Pig)
組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11574Oryctolagus cuniculus (Rabbit)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY11575Capra hircus; Caprine (Goat)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY11576Equus caballus; Equine (Horse)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11577Bos taurus; Bovine (Cattle)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11578Canis familiaris; Canine (Dog)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11579Sus scrofa; Porcine (Pig)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11580Oryctolagus cuniculus (Rabbit)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11581Rhesus monkey (Simian)
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY11582Bos taurus; Bovine (Cattle)
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY11583Capra hircus; Caprine (Goat)
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY11584Equus caballus; Equine (Horse)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。

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